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Taken together, these data show that dynamic modulation of [Ca2+]i occurs in WT cone photoreceptors and that this modulation is mostly retained in light-insensitive pde6c w59 mutant cones prior to their degeneration, although fewer frequently flashing cells were observed.
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In NOR Flash, cells are connected in parallel to the bit lines, which notably allow the cells to be read and programmed individually.
Sensory memory: Plastic flash memory cells were combined with a rubber pressure sensor.
For flash exposure experiments, cells were equilibrated to room temperature and then were exposed to a single flash from a commercial camera flash attachment (Vivitar).
The cells were flash-frozen in liquid nitrogen and stored at -80°C until use.
NAND flash memory cells are organized in an array→block→page hierarchy, where one NAND flash memory array is partitioned into blocks, and each block contains a number of pages.
At early exponential phase (Q1), 1.5 L of culture biomass (1 L from 1 reactor plus 0.5 L from a replicate reactor) were collected (10,625 x g, 10 min at 4 °C) for each duplicate, the supernatant was discarded, and the collected cells were immediately flash frozen in liquid nitrogen.
The residual cells were flash frozen and stored at −80 °C.
Cells were flash-frozen, thawed, and then lysed using sterile glass beads.
After washes in PBS, cells were flash frozen in liquid nitrogen.
Cells were flash thawed at 37°C, resuspended in R10 and centrifuged at 1400rpm for seven minutes.
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