Sentence examples similar to flash transcription from inspiring English sources
The sgRNA expression vectors were digested by DraI, and the sgRNAs were synthesized using the AmpliScribe T7-Flash Transcription Kit (Epicentre).
The selected 120 bp probes were subjected to in vitro transcription to produce digoxigenin (DIG -labeled cRNA with the AmpliScribe T7-Flash Transcription Kit (Epicentre, MaDIG -labeledSA).
Biotin-labeled CCAT1-L truncation probes were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and AmpliScripe T7/SP6-flash Transcription Kit (Epicentre).
After agarose gel electrophoresis, the PCR products were excised and purified with the QIAquick Gel Extraction Kit (Qiagen), and subsequently used as templates for dsRNA synthesis performed with the AmpliScribe T7-Flash transcription kit (Epicentre).
Total RNA (3 20 μg) was extracted from freshly frozen tissue using an RNeasy kit (Quiagen, Valencia, CA) and amplified using the AmpliScript T7-flash transcription kit (Epicentre, Madison, WI).
β-Catenin transcription was assessed using the TOP FLASH/FOP FLASH reporter luciferase assay.
All RNA samples were also analyzed for integrity and genomic DNA contamination using flash gel (Lonza, MD) prior to their use in reverse transcription.
We then performed β-catenin-regulated transcription (CRT) reporter gene assays by transfection of TOP-FLASH (wild-type TCF binding site) or FOP-FLASH (mutant TCF binding site) Wnt reporter plasmids into K562 cells.
Briefly, total RNA was isolated from 50 mg flash-frozen liver tissue using RNeasy-easy kit (Qiagen, Hilden, Germany, Cat: 74104), followed by reverse transcription of 1 μg aliquots of RNA (Fermentas).
Transcription errors were also common.
