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Thus, although Gnat1−/− Gnat2cpfl3/cpfl3 mice retain an ERG to a very high intensity flash, responses at moderate light levels (which are a feature of TKO mice) are lost.
Paired flash responses have been used successfully to determine the rate of recovery of photoreceptor currents in vivo, [23] [30], and are known to be reduced in patients with COD3 [15].
In the absence of Ih (Gh set to zero in the model), summation extends to delays above 600 ms. We tested the effect on Ih kinetics, band pass behavior, and flash responses, of recording in slices maintained near body temperature (∼35°C) instead of near room temperature (∼23°C) as in the above experiments.
The responses to moving stimuli were qualitatively similar to the flash responses shown in Fig 1A; there is a transient inward current as the stimulus enters the receptive field, a sustained inward current while the light intensity remains elevated, and an outward current with a concomitant reduction in current noise levels as the trailing edge of the stimulus exits the receptive field.
While brightb flash responses reversed polarity, dim flash responses became smaller but did not reverse.
MFA abolished both dim flash responses and bright flash plateaus.
Similar(23)
In response to males of her own species, of course, the female Photuris gives a flash response quite different from that of Photinus.
We instead exploited the predictive power of the simplified cellular model to look at the changes in the flash response caused by setting the Ih conductance to zero.
Thus, although band pass filtering by Ih reduces dim flash response amplitude, this is more than compensated by a sharpening of its time course.
Graph of the ratio of G over UV flash response amplitudes (brightb flashes delivered after a rod saturating pre-flash; spectral protocol) vs dim G flash response amplitude.
Spectral preference was quantified as the ratio of G and UV flash response amplitudes.
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