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Response amplitudes depend on the square of flash intensity, as expected for a chemical two-photon uncaging effect.
Experience suggests that even with a 100-fold increase in flash intensity, such concentrations would be difficult to realize.
HXP 120 Metal halide fluorescence illumination with intensity control.
Flash intensity is reported in nominal units.
At maximum flash intensity, the a-wave measure of photoreceptor hyperpolarisation has amplitudes of −28±5.8 µV or −31±3.5 µV, and implicit times of 61±7.6 milliseconds (ms) or 68±7.7 ms in control and drug-treated larvae, respectively.
Flash duration was 100 milliseconds for all intensities, with an interstimulus interval of between 10 seconds and 1 minute, depending on flash intensity.
The amplitude and latency (time to peak, or implicit time) of this deflection was dependent on flash intensity.
Two to 10 responses were averaged depending on flash intensity.
(B ) Rod responses to the same flash intensity at three different backgrounds.
Pictures were captured of the central cornea area using the automatic-mode low flash intensity.
The flash intensity was adjusted to produce occasional responses, as shown in the example trace.
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