Sentence examples for flash gel from inspiring English sources

Ligated fragments were amplified using adapter specific primers and size selected on Invitrogen's flash gel.

The DNA size and quantity was measured on a Lonza 2.2% flash gel (Lonza) and Quant-iT dsDNA high sensitivity kit (Invitrogen), respectively (data not shown).

All RNA samples were also analyzed for integrity and genomic DNA contamination using flash gel (Lonza, MD) prior to their use in reverse transcription.

The number of PCR cycles needed for amplification was determined by the ability to visualize the amplified product on a 2.2% Lonza flash gel.

DNA fragments were stained with 0.5 μg/ml ethidium bromide and were visualized under UV illumination using a Gene Flash gel documentation system (Syngene Bio imaging, UK).

The Nextera library product was quantified by picogreen, and fragment size distribution was checked by using Lonza flash gel, to ensure that fragment size distribution was between 300 1,000 bp.

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80.0 g extract was separated by flash silica gel column chromatography (400 g silica gel 60, 40 63 μm, Merck, VWR, Darmstadt, Germany; 41 × 3.5 cm) using a petroleum ether acetone gradient with an increasing amount of acetone.

Column chromatography (CC) was performed on flash silica gel (200 300 mesh; Qingdao Makall Group Co. , Ltd Qingdao; China).

The residue was purified by column chromatography (flash silica gel, DCM/EtOAc 99 1) to give the targets (1 18).

Organic layer was washed, dried over MgSO4, filtered, evaporated and purified by flash silica gel column chromatography using petroleum ether/ethyl acetate (80/20) as eluent system to obtain yellow solids, 2a in 92 % yield and 2b in 91 % yield.