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4) Protrusion increases dilatation, which means the FLAP signal is elevated on protruding segments.
Also shown is the computed FLAP signal at the end of bleaching (Fig. 4-A and -B).
Thus advective transport cannot be the reason for the increased leading edge FLAP signal in Case-1 vs Case-0.
The decrease in G-actin and F-actin at the leading edge is also important since the FLAP signal is inversely related to these quantities (see Eq. 9).
The FLAP ratio within each pixel is calculated by taking the local absolute FLAP signal and dividing it by the normalized signal of the reference fluorophore (CFP).
Given our analysis, the FLAP signal is highly sensitive to several parameters that characterize cytoskeletal dynamics in an exceedingly small compartment at the leading edge where crutial events are believed to happen.
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To the contrary, the simulations suggest a completely different explanation for the enhanced FLAP signals at the protruding boundaries and for the modulation of FLAP signals by myosin.
Once again, the simulation of FLAP experiments under these circumstances confirms the predictions of the dilatation hypothesis and provides an explanation for why FLAP signals are enhanced on advancing cell margins.
By allowing an improved treatment of effects related to the retrograde flow of the cytoskeleton and of the geometry and finite thickness of the lamella, this new analysis reveals a mechanism that can realistically explain the timing and the amplitude of all the FLAP signals observed in [1] without invoking special transport modalities.
The FLAP ratio signal thus avoids a number of complexities and artifacts that plague interpretation of the signals provided by simpler techniques like FRAP [21].
The developed active control scheme is local in that the flap control signal at a given longitudinal position along the bridge only depends on local motion measurements.
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