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Protein Blast was used to find protein coding genes homologue to the genes flanking with Pldi and Ak15880.
All the intact elements and solo Cassandra copies flanking with 5-bp TSDs were computationally verified to make sure that each of them contains the TSDs with one nucleotide mismatch allowed.
The TRIPZ control GFP vector was created by removing the turboRFP from the TRIPZ control vector by digestion with AgeI and ClaI and replacing it with GFP amplified with primers flanking with AgeI and ClaI restriction sites.
For example, genotyping of as few as 126 DNA markers would permit one to assess cosegregation at DNA markers flanking with 53% (159/300) of the RGAs in the cotton reference sequence.
The KAN-MX6, NAT-MX6, and LEU2 fragments flanking with homologous sequence to the target gene (40 bp) for deletion were amplified from the plasmid pFA6-KAN-MX6 (Longtine et al., 1998), pFA6-NAT-MX6 (Goldstein and McCusker, 1999), and pRS305 (Sikorski and Hieter, 1989), respectively.
A 2,6-dibenzimidazole-appended naphthalene derivative flanking with two N-alkyl chains (sensor 4) was designed and applied for highly sensitive detection of picric acid (PA) in aqueous media.
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His fellow minister, Ségolène Royal, was just along the corridor, flanked with the offices of ambassadors and high-ranking officials.
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