Sentence examples for flanking area from inspiring English sources

Removal of the downstream flanking area from the expression vector resulted in the 4-fold drop in the expression level.

A single PCR amplification of the entire coding region and the 3' flanking area of NAT1 (923 bp) using forward primer: 5'-tactgggctctgaccactat-3' and reverse primer: 5'-tgctttctagcataaatcacc-3' was performed.

We outlined a strategy allowing to address simultaneously 13 genetic variants in the coding area of NAT1 with two sequencing tracks: A and G and 11 variants in the 3' flanking area with two tracks: A and T (Table 1).

For leaf trichome density, three 57-mm leaf discs were collected from the flanking area of the main vein of the eighth leaf, treated, and observed (the adaxial surface or the upper side of a leaf) as previously described (Pomeranz et al. 2013) using a Leica MZFL III dissector with an additional polarized light filter.

In the TargetScan algorithm, the prediction score of a miRNA binding site depends on the level of conservation and sequence context criteria such as the distance of the target from the 3'UTR end and the AU composition of the flanking area.

Another pattern of SNPs was found correlated to the estrone level; the variant allele in the 5' flanking area of CYP11 in combination with the wt GSTT1 was present among 12 individuals with a plasma level of estrone above 68 pmol/l, while none of the individuals with E1 plasma level below 68 pmol/l carried this combination (CorrMAX 0.36 53/52) P-value 0.05).

It also should be mentioned that flanking areas of the SNpc, like the ventral tegmental area, have recently been reported to be involved in the regulation [16], and perhaps genesis, of REM sleep as we demonstrated for the SNpc.

Cloning the upstream and downstream flanking areas of the EEF1A gene into the pBL-2-ID-EBV plasmid resulted in the expression vector p1.1.

The development of the GoldenGate SNP markers from the Clementine sequence without information on the interspecific variability in flanking areas resulted in numerous homozygous null alleles in pummelo as described by Ollitrault et al. [ 8] and in trifoliate orange.

It was shown that presence of both flanking areas in the EEF1A-based vectors results in the 6- to 35- fold increase of the average expression level comparing to commercial vectors with CMV or EF1alpha promoters.

EEF1A-based expression vector includes 4.1 kb upstream and 4.2 kb downstream flanking areas of the EEF1A gene, so the ORF of the of the target gene replaces the coding exons of the elongation factor 1 alpha protein in the natural EEF1A gene, mimicking with all possible accuracy the structure of the natural gene in the resulting expression plasmid.