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Similarly, the N62 pool consisted of a 62-nucleotide (N62) region flanked by a different pair of primers: 5' - GATAATACGACTCACTATAGGCGCTCCGACCTTAGTCTCTG-N62-GAACCGTGTAGCACAGCAGA - 3.' Following in vitro transcription by T7 RNA polymerase (Epicenter Technologies, Madison, WI), the derived RNA library was purified on an 8% denaturing polyacrylamide gel, then eluted and precipitated.
It is interesting to note that although in E. histolytica Eh_ERE1 is flanked by a different type of TIR, the single copy of Ed_SINE1 identified so far in E. histolytica (Eh_SINE3) [ 2, 5] is inserted next to a truncated Eh_ERE1 element that carries the whole coding sequence of the element, resembling the organization of Ed_ERE1 in E. dispar (see below and Fig. 8C).
As suggested by Rowe-Magnus et al. [ 12], the fact that the core vibrio group (which includes V. parahaemolyticus and Vibrio sp. DAT722) integron integrases are flanked by a different gene than their V. cholerae and V. vulnificus homologs most likely represents an integron translocation event in the ancestor of the former group.
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All genomes contained a very large T6SS cluster as a component of the Cronobacter core genome, which is flanked by a highly variable region of different sizes in each genome containing several hypothetical protein CDS as well as those encoding numerous homologues of vgrG, Rhs-family and YD-repeat proteins (Table 4, T6SS7; Additional file 2: Table S2).
They were flanked by a black-cloaked choir.
It was flanked by a dozen security men on motorcycles.
And flanked by a police escort.
The agent was flanked by a huge German shepherd.
Several situations could be distinguished: 1) a position flanked by an STR on each side, of different period, motif, or phase was counted for both STRs.
Interestingly, the B. longum genome contains novel mobile integrase cassettes (MIC) consisting of three different contiguous integrases flanked by an inverted repeat and a palindrome structure sandwiched by two IS 3-type IS elements (Additional file 2).
Identification of Tn 4401 inserted at different loci, on different plasmids, and flanked by different 5-bp target site duplications indicates a frequent and dynamic process of transposition.
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