"What Sea Shepherd Australia is doing with Australian flagged vessels and Dutch flagged vessels down in the Australian Antarctic territory is outside of any sort of control of the courts in the United States," West said.
If the windows immediately adjacent to flagged regions were also flagged, then the regions were deemed to be homozygous.
To ensure spot quality, features and their respective background which were not uniform in pixel fluorescence intensity distribution in both channels were flagged (non-uniformity outlier flagging algorithm).
Only those spot were selected which contained only a small percentage (< 3) of saturated pixels, were not flagged bad or found absent (flags > 0), had relatively uniform intensity and uniform background [Rgn R2 (635/532) > 0.6] and were detectable above background (SNR > 3).
Nevertheless, it is unlikely due to a deleterious effect of the Flag epitope as non-Flagged versions of these suppressors gave identical results (data not shown).
First, reads longer than 50 nt flagged with Illumina's low quality flag "B" were removed from all datasets.
Unfortunately, we did not record the specific reason for each edit (e.g., originally missing, flagged as out of range, or flagged as being inconsistent with other variables), making it impossible to determine absolute counts of all potential error sources.
Much of this traffic is foreign flagged and on "innocent passage," under a Flag-of-Convenience, with a Crew-of-Convenience, and with lower safety standards.
It will also be a useful tool for researchers using flagged data to reduce random error in flagging and to target systematic error.
The nuclear staining observed by immunocytofluorescence is similar to that observed in COS cells transfected with flagged CCNL1 and revealed by an anti-FLAG antibody (Berke et al, 2001).
