Sentence examples for flag vector from inspiring English sources

To test this idea we expressed either FLAG-CUL4A, FLAG-DDB2, FLAG-H3.1 (positive control) or FLAG vector alone (negative control) transiently (∼24 hrs) in HeLa cells.

Second, we employ a FLAG vector, each element of which indicates whether or not a column in the coefficient matrix is a zero-quantized column.

For expression in mammalian cells nm23-H1 or nm23-H2 was cloned into Flag vector.

The average intensity was then determined for each cell, and normalized to control transfected cells (β-gal plus either empty FLAG vector or pSUPER).

There was approximately a 15 fold increase of Id2 MFI in Id2 F, S5A F, and S5D F transfected myoblasts compared to control (CON) and empty flag vector (EV F) controls (Supplement Figure S1).

To establish huLM-COMTKI cells, the huLM were stably transfected with the pcDNA 3.1 (FLAG) vector expressing the human membrane-bound isoform of COMT cDNA using Fugene 6 Transfection Reagent (Roche Applied Science, Indianapolis, IN) according to the manufacturer's protocol.

To screen microarray, whole-gene expression was normalized with results of flag vector-transfected cells (control).

The plasmid FLAG-Sp1 was constructed by inserting an Sp1 fragment amplified from pcDNA-Sp1 into NotI-XbalI sites in the pcDNA-FLAG vector.

The plasmid FLAG-RNF4 was made by PCR using specific primers from plasmid RNF4 and by subcloning the PCR products into BamHI and NotI restriction sites of the pcDNA-FLAG vector.

For the surface expression of hTIM-1, hTIM-4, mTIM-1 and mTIM-4, the coding sequences which start from right behind the signal peptide and last to the end were cloned into the pCMV3-SP-N-FLAG vector.

The pQBI25-HL4-FLAG vector used for expressing GFP-FLAG was generated as previously described [42].