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(c) Multichannel blot of cells transfected with PTPRS with a C-terminal Flag tag.
The CCT1 subunit was engineered to carry a hexa-histidine tag or FLAG tag in the internal loop region.
D. Linker dependency of membrane-displayed somatostatin without FLAG tag upon receptor activation.
Fluorescence intensity of yeast strains transformed with plasmids for membrane-displayed somatostatin without FLAG tag.
Plasmids for producing membrane-displayed somatostatin without FLAG tag (Table 2) were constructed as follows.
We used constructs expressing a C-terminal FLAG tag for most of our experiments.
Similar(26)
Furthermore, the subunit 8 protein could be detected using anti-FLAG tag monoclonal antibody.
This was done by indirect detection using the tri-FLAG tag present on all formats.
Anti-Flag tag and anti-HA tag antibodies were purchased from Sigma-Aldrich.
FAAP24, MHF1, Rad51, and Rad52 were subcloned into pBabe-Puro, with 3× N-terminal Flag-tag.
A rapid and simple purification strategy was established by incorporating a FLAG-tag at the C-terminus of the protein.
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