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The flag leaf length (FLL, cm) and flag leaf width (FLW, cm) were measured on three tillers.
Flag leaf length was measured as the length of leaf-bearing primary panicle from leaf base to the tip.
Likewise, the population averages of flag leaf length (FLL) and flag leaf width (FLW) were only slightly reduced.
The starch synthesis related gene markers, SSI, SSIIa, SBE1, AGPL4, and ISA1, were linked to plant height (PH), panicle length (PL), flag leaf length (FLL), GW, and GLW.
Four QTLs were identified for flag leaf length under SF on chromosomes 4, 5, 6, and 9 (Table 3, Fig. 1).
Wang et al. (2011) narrowed the location of qFL1 for flag leaf length to a 31 kb region containing 4 predicted genes with BC2F3 and BC3F2.
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In our studies, the Dw1-Dw4 reportedreported by Morris et al. [ 4] show the most striking signals associated with base-flag leaf length for two years (Additional file 2: Figure S12).
Plant data collected in the field included: flowering time as days to anthesis, plant height (cm), upper leaf angle (at the leaf subtending the flag leaf), leaf length and width (cm), tassel length (cm), tassel branch count and angle; stem puncture resistance (rind penetrometer resistance), a measure of stalk strength; and stem width (cm).
Data were recorded on ten plants from each entry and replication for agronomic traits like plant height, tillers/plant, panicle length, number of filled grains/panicle, 1000-grain weight, flag leaf, 2nd leaf length and breadth while days to 50% flowering was recorded on whole plot basis data analysis was performed using SAS statistical software (SAS Institute Inc. 2010).
A genomic region on chromosome 4 was detected with multiple effects on increased flag leaf width and length, and panicle number and length in rice [ 11].
Data were collected on canopy temperature, relative growth rate, green flag leaf area duration, assimilates translocation and grain yield.
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