Sentence examples for fixing solution from inspiring English sources

Fixed root tips were stored at −20 °C in fresh fixing solution.

Then, the pre-fixed rings were removed from the apparatus and further fixed in the same fixing solution for 2 h at 4 C with agitation.

Further, the fixing solution was aspirated and traces of fixing agents were removed by rinsing several times with PBS.

Then, the fixing solution was removed and the cells were stained with 1% crystal violet in 3.7% formaldehyde.

An equal volume of fixing solution (0.9% NaCl, 3.7% formaldehyde) was added to 500 µl aliquots of cells.

The brains were then dissected and post fixed with the same fixing solution overnight at 4°C.

The media were removed and cells were fixed by 2 ml fixing solution (1% formaldehyde, 0.2% glutaraldehyde in PBS) 48 hrs after infection.

Cells were washed twice with 1 ml BD perm/wash solution (1×), and resuspended in fixing solution before analyzing by Becton Dickinson FACSan.

The fixing solution was rejected and 100 µl/well anti-BrdU-POD antibody solution was added to bind the incorporated BrdU.

After washing in PBS containing 0.1% Tween 20, samples were incubated in 1 µg/ml ProteinaseK for 18 minutes, and refixed with fixing solution (4% PFA/0.2% glutaraldehyde) for 10 minutes.

We also believe that the relatively small amount of cellular material in each well allows rapid quenching of treatments and preservation of the cell-interior by simple addition of fixing solution.