In this case, E. coli DSS640 may be considered a poor biofilm former in minimal media, a good biofilm former in rich media, or mistaken for a hyper-biofilm former when treated with an alternative fixing protocol.
Considerations for setting up microscopy analysis included the fixing protocol, choice of growth medium, viable cell counting, cross-talk between fluorophores, as well as the method of 3D visualization.
When grown in rich medium and examined using a standard fixing protocol, biofilms of E. coli TG1 (wild type) and DSS640 (tatABC - ) similarly produced surface-adherent layers of cells that had little EPS, and similarly, were thickest near the air-liquid-surface interface (Fig. 2E and 2F).
The optimal protocol also outperforms the fixed protocols by 50%% on average.
The philosophy of this pattern is to reduce recurrences while administering less injections and requiring less visits than fixed protocols.
Overall, a guideline standardized method does not currently exist, and therefore all previous studies employed fixed protocols.
Finally, a medical practitioner examined all the checklists in order to evaluate and amend them if deemed necessary based on pre-arranged and fixed protocols.
