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For larger and more established organizations, "fixing" culture after the fact is no easy feat.
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After glutaraldehyde fixing, cultures were lifted, mounted on slides and stained with May-Grundwald and Giemsa stains then visualized under the microscope.
"Our tool doesn't fix culture.
Matching formalin fixed cultures were analyzed by the EGFR-SRM assay and benchmarked against immunoassay of the non-fixed cultured cells.
Before scoring for morphology, fixed cultures were briefly sonicated in order to separate clumps.
In a first set of experiments, we fixed cultures at different time points after stimulation with 10 light pulses and stained for c-Fos (Fig. 6B).
For optical clearing, fixed cultures were processed according to the method described (Yang et al., 2014).
Invasion densities were quantified by counting fixed cultures under transmitted light using an Olympus CK2 inverted microscope equipped with eyepieces with a 10 × 10 ocular grid.
Treated and fixed cultures were immunolabelled with an anti-activated caspase-3 antibody and 6 images were acquired from randomly selected fields of view of the axon compartment on a Leica (DM LB2) fluorescent upright microscope with a 40× objective.
To assay for centriole disengagement/reduplication we fixed cultures 48 and 72 hours after transfection and immunostained for a number of recognized centriolar proteins and modifications to centriolar microtubules.
And he promised that the agency would achieve the major change called for in the report, fixing its culture to one more amenable to safety concerns.
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