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After establishing that, in some situations, stronger fixation conditions enhanced the cytoplasmic versus cortex staining patterns, we next decided to investigate PADI6 and MATER expression in embedded ovarian and oviduct cross sections with the idea that by both fixing and embedding the oocytes, CPL-associated proteins would remain fixed in their correct positions.
After fixing and embedding of the tissue in paraffin, thin sections were cut and stained with H&E using standard methods.
The intensity of staining may not be only because of the variation of kallikrein expression in each pathological specimen, but also be contributed by the variations in fixing and embedding procedures used during the processing of tissues.
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Then scaffolds were fixed and embedded for light microscopy and electron microscopy by routine methods.
This microtome is used to section tissue that has been fixed and embedded in either paraffin or resin.
Cells were infected with MHV-68, fixed and embedded.
Liver samples were fixed and embedded in paraffin then sectioned for immunohistochemical analysis.
Tissues were fixed and embedded in paraffin.
Lungs were collected, fixed and embedded into paraffin.
The eyes were enucleated, fixed, and embedded in paraffin.
Kidneys were removed, fixed and embedded in paraffin.
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