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Briefly, this volume was prepared from a hippocampal slice of P21 male rat of the Long-Evans strain, fixed via perfusion through the heart with glutaraldehyde fixative, and then processed with potassium ferrocyanide-reduced osmium, osmium, and aqueous uranyl acetate.
To ameliorate this issue, we acutely sliced brain sections of 250 µm thickness (using standard brain slicing procedures [ Atasoy et al., 2012] for electrophysiological recordings), and these were fixed via submersion in 4% PFA in 0.1 M phosphate buffer fixative (pH 7.4) for 1-hr at room temperature.
Different from other periodic cellular materials, novel transformed pattern for SMP material can be fixed via cooling to a temperature below the glass transition temperature Tg, and this fixed pattern can further be recovered to its original pattern by reheating to a temperature above Tg.
The problem was fixed via a software update.
The game launched with a number of irritating bugs, and although many have been fixed via downloadable patches, a handful still remain.
A reproducible 3-part fracture was created in paired human cadaveric bone and then fixed via the locking screw implants.
It was found that the self-organized nanophases were formed in the mixture before curing reaction and the nanostructures can be further fixed via curing reaction.
After a 2-week survival period the animals were killed, fixed via transcardial perfusion, and the retinas whole mounted and examined under fluorescent excitation to reveal the pattern of retrograde transport.
i had it sat & fixed via restart (but not working for others).
Obviously, developing for a client app that can't be fixed via a push to AWS has its own challenges.
Power Now: Enabled, but frequency fixed via On Demand governor and Core Performance Boost disabled.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com