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An allograft bone graft or autogenous iliac bone graft was then implanted and fixed using an anterior plate.
Full-thickness cartilage defects of 8 mm in diameter were created in the tubular bone condyle of the fetlock joint and cartilage grafts were fixed using an anchor system, while defects without grafting served as controls.
(3) when C i equals to 3 3.1 When the atom is N or P, and if it connects to oxygen (A j ) and C j = 1, this group is fixed using an acid model.
In contrast to previous studies where the osteotomy was fixed using an external fixator [5], we have used plates and screws to avoid tethering of the quadriceps, pin track infections, loosening and the use of a cumbersome device.
To avoid bone tunnels on the femoral side and improve the stability of fixation, the graft is fixed using an 5.5-mm diameter titanium screw-type anchor, normally used in shoulder surgery, loaded with two strands of no. 2 nonabsorbable suture, and occasionally, additional trans-osseus sutures are performed.
Red blood cells were lysed and leukocytes fixed using an automated EPICS Q-PREP system (Coulter Electronics, Hialeah, FL).
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Briefly, cells were washed with HBSS, fixed using a fixative solution for 10 15 minutes, again washed with PBS, and then incubated with Staining Solution Mix overnight at 37°C.
Wax sections of limbs were fixed using a zinc based fixative [12], [13] for 48 hrs at 4°C, followed by 48 hrs in 50% ethanol.
The cells were then trypsinised and subsequently fixed using a combination of both Carnoy's fixative (acetic acid/methanol 1∶3) and three to four drops of formaldehyde (to fix the cytoplasm).
An. gambiae embryos were fixed using a comparable procedure, except that eggs were fixed at room temperature.
That, they fixed using a patch from a third species, Janthinobacterium.
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