Sentence examples for fixed treated from inspiring English sources

Tissue was fixed, treated with RNase, stained with PI and examined by MPLSM.

Adult male brains were dissected, fixed, treated with primary and secondary antibodies, and prepared for confocal imaging as described previously [68].

Cross- and longitudinal cryosections made from fixed treated specimens revealed that internalization is carried out by the ectodermal cell layer, facing directly the medium (Figure 7).

Briefly, cells were fixed, treated with RNase A and stained with PI 40 μg/mL, then subjected to flow cytometry analysis.

17 For the detection of IE1/2 and UL99 antigens, cells were fixed, treated and incubated with a blocking buffer as described previously.

For Figure 2A, cells were fixed, treated with osmium, embedded in Epon, indirectly immunolabelled on ultrathin sections, contrasted with uranyl acetate, and digital images collected on a Zeiss 912 Omega electron microscope [ 39].

Anti-FIX IgG Abs measured by ELISA (A, B, E, F) and FIX inhibitors measured by Bethesda Assay (C, D, G, H) in plasma samples collected from LV-FIX treated mice at the indicated times after FIX immunization.

Tregs isolated from LV-FIX treated mice suppressed the proliferation of FIX-specific effector T cells while showing only modest activity on OVA-specific effector T cells stimulated by the cognate Ag.

Whereas saline-injected mice (n = 5) showed stable high concentration of anti-FIX IgG (>250 μg/ml) and inhibitors (4 BU/ml) throughout the follow-up time, LV-FIX treated mice (n = 10 in 2 independent experiments) showed an initial surge followed by a sharp decline of both anti-FIX IgG and inhibitors, which decreased to <10 μg/ml and to undetectable levels, respectively.

We also found a consistent decrease in anti-FIX IgG1, IgG2a and IgG2b in LV-FIX treated responders mice as compared to saline-injected controls both after early and late treatment, further supporting effective reversal of anti-FIX humoral immunity (Supporting Information Fig S3).

The CCI received by the cell under consideration is fixed and treated as an additive noise.