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Traditionally, scientists have treated the laws of physics as simply "given", elegant mathematical relationships that were somehow imprinted on the universe at its birth, and fixed thereafter.
Yet, a different type of AChE1 insensitivity was described 10 years ago in a Culex pipiens population collected in Cyprus in 1987 and fixed thereafter as the ACE-R strain.
PBMCs were immediately fixed thereafter using fixation/permeabilization buffer (True-Nuclear-kit, BioLegend) according to manufacturer's instructions.
For the MI and PI subgroups, the rates of SSI receipt increase slightly until the application period and basically remain fixed thereafter.
Cells treated with 25 ng/mL Emmprin or 100 µM deferoxamine (DFO) for 4 hours were immediately fixed; thereafter subjected to in situ PLA using Duolink Detection kit (Olink Bioscience, Uppsala, Sweden) according to the manufacturer's instructions for Duolink Blocking solution and Detection protocol.
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In paragraph (1)(H), the words "functions which by law expire on the termination of present hostilities, within any fixed period thereafter, or before July 1, 1947" are omitted as executed.
For BrdU (Sigma-Aldrich) staining, cells were labeled by incubation for 12 hr after serum starvation with 50 nM BrdU dissolved in 10% FBS/DMEM; and the cells were then fixed and thereafter incubated in HCl to denature the DNA.
A part of infected explants were given a single pulse of EdU for 24 h between days 1 and 2, and fixed immediately thereafter, at 2 DIV.
Sometimes I realize I've shelved something incorrectly, and fixing it thereafter feels somehow like a medical relief drugs for dorks.
The left lung was weighed and thereafter fixed in 4% formalin and embedded in paraffin.
After a 24-h incubation, samples were washed in PBS 0.1 M, trypsinized and thereafter fixed with 1% w/v glutaraldehyde 4% w/v paraformaldehyde in PBS 0.1 M pH 7.2 for 2 h at 4°C.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com