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The OPLC says that while fixed slides and flumes will not be possible in the aquatics centre, they will use portable, inflatable equipment to attract leisure users to the pools.
Formalin fixed slides were dewaxed with xylene, then rehydrated through a graded series of alcohols and distilled water.
For the staining of livers for accumulation of lipids fixed slides was incubated 8 min. in pre-warmed (60°C) Oil Red O solution (0.5% in propylene glycol), destained 2×5 min in propylene glycol and rinsed twice in water.
The fixed slides were labeled using a TUNEL assay kit according to the manufacturer's instructions (Roche).
Immunocytochemical staining of deparaffinized, fixed slides was performed after antigen retrieval by microwave heating in citrate buffer.
To ensure visualization of cytoskeletal proteins, fixed slides were permeabilized with 0.1% Triton X-100 in PBS for 10 min when necessary.
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The heat fixed slide material was emulsified using a sterile swab and sterile distilled water.
This avoids the extra step for merging neighboring enriched regions with a fixed sliding window-size.
For immunofluorescent studies, cells were spread and fixed on slides using Cytology Fixative (Surgipath, Richmond, IL, USA).
For tumor tissue fixed on slides, diluted proteinase K was placed on the targeted tissue section area of the slide.
The infected and mock-infected monocytes were harvested and fixed on slides using cold acetone for 10 minutes.
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