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For scanning electron microscopy (SEM), Ehrlichia-infected DH82 cells were cultured on Thermanox coverslips (Nunc, Rochester, NY) and were fixed similarly as for TEM, dehydrated in ethanol and processed through hexamethyldisilazane followed by air drying and mounting on stubs.
B. subtilis Spo0J cells were harvested and fixed similarly as mentioned above.
In contrast, the circular genomic permutation, which is also performed at the SNP-level where the number of significant genes in the pathway and in the universe are not fixed (similarly to the SNP-level random permutations), seems to capture the genomic structure and shows how likely an outcome is expected according to this structure.
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Following the release of its first-ever earnings report in December, shares of Stitch Fix similarly took a huge hit, plunging down 10percentt on the news.
We implement this method using fixed effects in both the 'trimming' and 'filling' parts of the algorithm (fixed-fixed), similarly, random effects for both parts (random-random), and fixed effects to 'trim' and random effects to 'fill' (fixed-random).
Joining the single currency means adopting what a Chinese Communist might call "the three inflexibilities": an interest rate that cannot be varied to match the conditions of the national economy; an exchange rate that is similarly fixed; and the strict fiscal rules of the stability pact.
The lawn was fixed and permeabilized similarly to IHC tissue.
After the desired incubation time (6, 24, and 72 h), the cells were fixed and stained similarly as described in [28, 29].
The top diameter (D) to periodicity (P) ratio (D/P ratio) of the SiNCs is fixed at 0.5, similarly based on the experimental value, while P is varied from 200 to 800 nm to find the optimal structure that maximizes sunlight absorption.
It would be sad if today's renewed preoccupation with American exceptionalism ends up sacrificing the freedom it purports to be preserving for a similarly fixed and incurious vision of the world.
DCs untreated or treated with mPE for 48 hours were similarly fixed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com