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Of course it's formulaic, just as the Observer newspaper is formulaic, built around fixed sections and regular columnists.
So we realised that people are interested in paraffin fixed sections".
This is because the number of fixed sections on moving objects with greater strength will protect users more effectively.
We validated the technique on human cardiomyocytes either freshly isolated or selectively excised from fixed sections of human myocardium by Laser Capture Microdissection.
There was no apparent effect on morphology in the GAD-ir neurons, although in fomaldehyde fixed sections it is difficult to distinguish processes.
Briefly snap frozen acetone fixed sections were blocked and then incubated with the goat anti human C4BP (1∶50 dilution) and mouse anti human CD40.
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Immunohistochemistry was performed on 4 μm paraformaldehyde fixed sections.
LV cross-sectional area was measured on formalin-fixed sections (ImageJ, National Institutes of Health).
Formalin-fixed sections were processed by the Cleveland Clinic Histology Core.
For CD73 immunostaining, HOPE-fixed sections were deparaffinized in isopropanol at 60°C and rehydrated in 70% acetone.
For terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) analysis, formalin-fixed sections were deparaffinized, rehydrated, and pretreated with proteinase K. Apoptotic cells were detected using DeadEnd Colorimetric TUNEL system kit (Promega, Madison, WI) according to the manufacturer's instructions.
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