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In this work, we carried out SVGs with unfixed and fixed samples.
Fixed samples were dehydrated by exposure a graded series of water-ethanol mixtures.
Fixed samples were dehydrated in graded ethanol series, clarified in xylene and embedded in Paraplast (Sigma-CO).
In addition to preventing damage to fixed samples during STED microscopy, in the future guided STED should be well suited for live cell imaging.
The fixed samples were stained with rhodamine-phalloidin to visualize F-actin and 4′, 6-diamidino-2-phenylindole (DAPI) to visualize cell nuclei.
Fixed samples were washed with 0.1% triton X-100 in PBS then blocked with 5% normal goat serum in 0.1% triton X-100 in PBS.
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Actin in TCA-fixed samples was detected with a goat anti-actin antibody.
Pathologists were unable to determine extrinsic tongue muscle invasion in post-formalin-fixed samples.
We analyzed frozen and paraffin-fixed samples of tissue obtained from brain donors diagnosed with AD and control individuals.
The alcohol-fixed samples were processed in an Autotechnicon tissue processor or in an incubator.
Betge J, Kerr G, Miersch T et al. Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples.
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