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Slides were prepared as acetone fixed preparations of the purified antigens by experienced laboratory technicians capable of maintaining all conditions equal between test runs.
However, immunostaining of the whole Drosophila testis is often associated with insufficient resolution at the subcellular level, poor reproducibility, and incomplete staining of fixed preparations.
We have followed mitotic spindle establishment by time-lapse microscopy and on fixed preparations.
For the smear test, fixed preparations were stained with auramine and visualized under a fluorescence microscope (at × 400 magnification).
Procedures used for imaging living or fixed preparations of cultured embryos are given in the Extended Experimental Procedures.
In general, imaging of these fine subcellular domains in live samples produced clearer and more consistent localization patterns than fixed preparations, leading us to use the live imaging approach whenever applicable, throughout the analysis.
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Acrylic and bisacrylate resins are the most indicated to temporarily seal the fixed prosthodontics preparations; however, for partial preparations on posterior teeth, the temporary composite resin is also indicated [2].
Meanwhile, the focus of national attention remains fixed on preparations by the leading political parties for the election battle ahead.
Using 13 fixed cadaveric preparations, we sought to design a surgical approach that would cause minimal involvement of the surrounding structures and maximal exposure to the extreme high cervical area.
Dual-arch impression trays often are used for addition silicone final impressions of fixed prosthodontic preparations, but concerns about distortion of the impression are common because such trays lack rigidity.
We created fixed cell preparations from nocodazole-treated (high cyclin B1) and serum starved (low cyclin B1) Pcellslls that were either lyophilized (for preservation) or solubilized.
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