Sentence examples for fixed parasites from inspiring English sources

Here we show both qualitatively and quantitatively that compared to air-dried and briefly fixed parasites, longer fixation in suspension leads to improved conservation of nuclear structure and lower intra-population variation of nuclear shape as well as area after FISH development.

Hoechst 33258 (Molecular probes) was used in a concentration of 50 ng/ml for fixed parasites or 10 µg/ml for live parasites.

As illustrated in figure 2C, immunostaining whole fixed parasites using anti-SmAQP antiserum further confirms the robust knockdown of SmAQP protein.

For staining fixed parasites with the antibodies secreted from hybridomas, culture supernatants were collected and used undiluted on intracellular N. caninum essentially as described for similar assays in T. gondii [27], [28].

Following transfection, subcellular localization was determined by direct fluorescence imaging of YFP (Fig. 2 left), or staining of fixed parasites with antibodies specific for native microneme proteins, rhoptry proteins, and either YFP or the HA epitope tag (Fig. 2, right).

The fixed parasites were also incubated with either a MSP119-specific rabbit polyclonal antibody or an antibody specific for the CRT protein, and the binding of these antibodies was detected with appropriately labeled second antibodies.

Immunocytochemistry was performed on the fixed parasite material using the primary antibodies rabbit anti-GFP (ABCAM, USA, used at 1 in 200dilution) and mouse monoclonal anti-alpha tubulin (Sigma, UK, used at 1 in 500).

The presence of antibody recognizing P. falciparum CS protein in its native configuration was determined by an indirect IFA method [20] using NF54 parasites fixed on 10-well slides, 5000 sporozoites/well (kindly provided by C. C. Hermsen, NL-Nijmegen).

The coverslips were washed for the removal of extracellular parasites, fixed with Bouin overnight and stained with Giemsa (Laborclin, Pinhais, PR, Brazil).

Tissues were delivered fresh to Evira, where they were dissected under stereo microscope for adult parasites, fixed in 10% neutral buffered formalin and routinely processed; embedded in paraffin, cut in 4 μm sections and stained with haematoxylin and eosin, and examined histopathologically.

A low homing frequency might be sufficient to provide purifying selection for the HE function, but insufficient to fix the parasite in the whole population simultaneously.