Sentence examples for fixed material from inspiring English sources

Following blocking, primary antibodies were diluted in blocking solution and cells were incubated with primary antibody solution overnight at 4 °C except for the γH2A.X antibody which was kept for only 2 h at room temperature on the fixed material.

However, alternative preparative methods provided independent evidence for the validity of at least some of the cellular EM that was being done on fixed material.

In addition, so-called target schedule constraints need to be considered, such as fixed material delivery dates, on-site resources, contracted milestones and established sequences.

They also provide mechanistic hypotheses not foreseeable from fixed material, or homogenous population extracts.

Ultrastructural analysis was performed on muscle tissue retrieved from formalin fixed material.

Sections of 100-nm thickness from fixed material were cut and mounted on gold grids.

The PFA-fixed material was routinely embedded in paraffin.

Formalin-fixed material from brain and spinal cord was routinely embedded in paraffin.

With paraformaldehyde-fixed material, heat-induced antigen retrieval is beneficial for most antibodies.

Furthermore, application of the new methods required less time compared to formalin-fixed material.

Nucleic acid segments larger than 200-bp cannot be reproducibly amplified from formalin-fixed material [ 17].