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All other nine morphological parameters on colony level (like colony ecological volume, total number of branches, final height etc., Table 1) were kept fixed (major outcomes are shown in Fig. 5).
Genotype at rs1020004 or rs1990622 for the 21 patients and 29 unaffected carriers was entered in the trait model as a fixed major gene covariate (Wijsman and Yu, 2004).
Two-point linkage analyses for QTLs influencing either QT1 or QT2 were conducted under a fixed major gene model which assumed a two-allele QTL (alleles A and a), genotype-specific means, μAA = – 1.9, μAa = 0.5, and μaa = 2.5, frequency of allele A (associated with lower trait values) = 0.75, and a common within-genotype variance, σ2 = 0.5.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com