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A hybrid OR incorporates fixed imaging in a sterile OR setting.
Therefore, fixed imaging yields a mixture of oocytes that have been arrested for varying amounts of time.
…the movies that have been performed on ICAM-1 coated slides (unlike the fixed microscopy that was performed on uncoated slides)… In fixed imaging ICAM-1 interactions are provided by the target cells themselves, with conjugates formed in suspension before plating.
We therefore think that definitively answering this question will not be possible using fixed imaging, and would be better determined by high resolution live imaging, where timepoints within the same spindle can be compared.
When acid treatment was applied, cells were incubated for 5 min at room temperature with acid solution (0.1 M Na-glycine pH 3.0, 0.5 M NaCl) [ 28- 30] and then fixed Imaging was performed in PBS.
In non-ECG-synchronised CT in paediatric patients a fixed imaging delay is usually sufficient with the start delay set at 12 s for peripheral injection and 8 s for central venous injection.
Similar(50)
Compared to traditional fluorescent dyes and proteins, modified quantum dots and gold nanoparticles possess alternative properties that enhance their imaging capabilities in cells that are fixed before imaging.
The device included a fixed focus imaging system and a linear motion system.
A human skull served as the matrix into which the typodont was fixed for imaging.
In some cases, cell cultures expressing CFP fusions were fixed before imaging in 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA 19440) and washed several times in PBS containing 0.05 M glycine before mounting with a polyvinyl alcohol-based medium.
Then, cells were washed with acid wash buffer and fixed for imaging.
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