Sentence examples similar to fixed freezing from inspiring English sources

With Auguste Deter's brain tissue fixed, frozen, sliced, stained and pressed between two thin pieces of glass, Alzheimer put down his habitual cigar, removed his pince-nez, and peered into his state-of-the-art Zeiss microscope.

Polar codes are generated by source bits including variable information bits and fixed frozen bits.

(c) Quantitative assessment of proliferating index of tumor cells in relation to RO5126766 treatment based on IHC staining of Ki-67 antigen of excised, acetone fixed frozen tumor tissue (*p < 0.05, n = 3).

With fixed frozen bits u F, where F=[ 1,L]∖A L (i), message vectors ({ {u_{1}^{L}}(i) }) are stacked in the N×L message matrix U, which is then encoded with the L×L generator matrix G L and output codewords matrix X.

Immunofluorescence was performed on 40 µm (brain and spinal cord) and 20 µm (gastrointestinal tract) paraformaldehyde fixed frozen sections.

Fixed frozen 10 µm pancreas sections from 8-week old mice were immunostained with a rat monoclonal anti-MECA-32 antibody (1∶200; BD PharMingen, San Diego, CA) to label vessel endothelial cells in the presence or absence of a rabbit polyclonal antibody to the NG2 pericyte marker (1∶200; Chemicon/Millipore).

Acetone fixed frozen sections of acute tissue and tissue from 29 to 38 days post contact infection were labelled in duplicate with FMDV capsid MAbs, with consecutive sections labelled with MAbs 2C2, 3C1 [11], [11] and isotype control MAbs TRT1, TRT3 [38] and AV29, a MAb directed against a chicken antigen provided by F Davison, IAH.

LCM was performed as described previously [32], except that ethanol fixed frozen sections were stained with a 1% solution of toluidine blue (Fluka) for 3 minutes, washed for 30 seconds in nuclease free water, dehydrated by a graded ethanol series and allowed to air dry for 10 minutes.

The mice were sacrificed by CO2 asphyxiation, and samples were immediately fixed, frozen, or processed for further analysis.

For the human LN analysis, cold acetone fixed frozen sections (cat#. T1234161) were purchased from BioChain Institute , Inc (Newark, CA).

In some cases, fixed frozen limbs or sterna were trimmed sequentially from both sides to expose the central medullar cavity and processed further for whole-mount fluorescence staining.