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Just fixed discs with no shift in them.
After rinsing in PBS, fixed discs were permeabilized in PBS containing 0.1% Triton X-100 (PBST) and then blocked for 30 60 min in 1% BSA in PBST.
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Immunostainings were performed by dissecting imaginal discs from wandering third instar larvae, fixing discs in 4% paraformaldehyde + PBS, followed by blocking in PBS + 0.1% Triton-X + 5% normal goat serum (NGS), incubation with primary antibodies overnight at 4°C, and incubation with secondary antibodies overnight at 4°C.
The clones were generated at the beginning of the ablation period and fixed the discs at different times after the end.
A newly developed fixed bed disc reactor (FBDR) which combines biofilm formation on biofilm carriers and reactor agitation in one single system was assessed for its applicability to demand-driven biogas production by variable feeding of sugar beet silage.
Mehra and Wolford [ 18] first inserted only one mitek anchor into the condylar process and fixed the disc with special suture in the treatment of 105 patients (188 joints), and achieved a good therapeutic effect.
To measure autofluorescence of eye disc samples, we fixed and imaged wildtype discs using the same conditions as for Yan-YFP discs.
In other words, end the cover disc is fixed and the central disc is used as output component.
BioCoat™ Osteologic™ Discs fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2 (buffer A) for 1 h from bone resorption assay were washed three times in buffer A, postfixed with 1% osmium tetroxide in buffer A, then dehydrated in graded ethanol.
Samples were fixed on metal discs with double-sided adhesive tape.
The clones were induced at the beginning of the ablation and the discs fixed at the end of it.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com