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Under such conditions, the ITO material cannot grow along the fixed crystal, so the NWs cannot be fabricated.
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We checked whether Lz+ cells increase in number throughout third instar larval development or if their number is fixed and crystal cells mature from these precursors.
From the same experiments as in Figure 2C, we photographed the cancer cells before they were fixed for crystal violet staining.
Chamber inserts were rinsed with PBS and then cells were stained and fixed with crystal violet/formalin mixture (Chemicon) for 20 minutes.
After another 24-hour culture, the number of cells that migrated to the lower surface was fixed by crystal violet and examined.
Cells were seeded the day before treatment (1500 cells/96-well cells/96-wellcubated for 72 h with platebulysin or tubulysin andThe medincubatediscarded, cells washed with PBS, stained and fored with crystal violet/0.5% methanol for 10 min.
After allowing agar to solidify, the plates were incubated for ∼60 hrs at 37°C before fixing with crystal violet and counting plaque number at each dilution.
After no more than 24 h, cells were fixed, stained with crystal violet and five pictures per condition were taken.
For the beamlines of the new synchrotron radiation source PETRA III, fixed exit double crystal monochromators with specific features were developed.
To keep a sample orientation, we fixed the zircon crystal sandwiched between the Transparent Tapes from NRM measurement to end of demagnetization/remanence measurement processes.
During the optimizations, alpha carbon atoms and nitrogen atoms attached to the beta carbon atoms of His546, Asn52, Tyr220 and Asp222 were kept fixed to their crystal structure positions to mimic the steric constraints by the protein matrix.
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