Briefly, cells were fixed onto chamber slides using 0.1 M phosphate buffered 2.5% glutaraldehyde, pH 7.4 overnight.
For staining of the 3D culture, the spheroids were fixed in chamber slides with 4% paraformaldehyde for 30 min at room temperature before permeabilization.
Renovation will include fixing drainage chambers; replacing deteriorating pavement with new bluestone and hexagonal asphalt blocks; adding new benches, drinking fountains and bike racks; renovating the fence; and adding bronze plaques bearing an artist's rendition of the Manhattan skyline as it appeared in the 1950's.
The filter inserts were removed from the chambers, fixed with methanol for 5 minutes, and stained with Harris Haemotoxylinn for 20 minutes.
To detect KIF5B on mitochondria, purified mitochondria were incubated with Atto550-labeled KIF5B, transferred into flow chambers, fixed and stained with antibodies.
iPSC‐derived DAn were grown on plastic cover slide chambers, fixed with 4% paraformaldehyde, and then permeabilized with 0.5% Triton X‐100 in TBS.
It's an entirely closed vessel, meaning that you can't bust in and fix a chamber or mouth piece, but that's likely what makes it so glamorous.
EGFP+ and EGFP− cells in the upper chambers and bottom chambers were fixed and stained with DAPI, and quantified under a fluorescence microscope.
Individual coverslips were transferred to a recording chamber fixed to the stage of the microscope fitted with a × 40 water-immersion lens.
Even after the chamber fixed its systems, a printer in one of its offices randomly started printing Chinese characters, and the group discovered that a thermometer in a chamber-owned apartment was communicating with an I.P. address in China.
