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We determined that traditionally used protein blocking steps are unnecessary in the immunostaining of routinely fixed cell and tissue samples.
In addition to the relatively fixed cell types described above, there are free cells that reside in the interstices of loose connective tissue.
This prompted us to explore whether commercially available Abs have a propensity for random non-specific binding in the immunolabelling of routinely fixed cell and tissue samples.
The fixed cell suspension was spotted on ethanol cleaned slides and air dried.
The stark contrast between live cell and fixed cell images illustrates hitherto unsuspected limitations to the fixation process.
RNA was extracted from ethanol/phenol fixed cell samples.
Similar(17)
We have used fixed cell-geometry-parameters for all the species and for all the drugs.
The n PRS takes fixed cell-specific values.
We used fixed cell-based immunoassays that allowed us to analyze many conditions simultaneously.
Here, automated detection of mitotic cell rounding was used to trigger live-to-fixed cell imaging protocols.
Chromatin in formaldehyde-fixed cell lysates was sonicated to an average size of 500 bp.
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