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Furthermore, most of the equipments of fixed blocking system and quasi-blocking system are based on single-configuration once single-configuration onceion isignalingced by faultsystem whole normal operating process would be severely affected.
Non-independence attributable to multiple measurements on different replicates was therefore dealt with by treating 'replicate' as a fixed blocking factor.
A fixed blocking matrix [31], which subtracts adjacent equalized and time-aligned microphone subband signals, is not suitable for belt microphones due to the strong microphone SNR variations.
The country where the trophy was offered was entered to all models, given established price differences [2]; country identity was entered first to each model as a fixed blocking factor.
Similar(56)
The podocytes then were fixed, blocked and stained with FITC-labeled phalloidin and DAPI and mounted for microscopy.
Cells were then fixed, blocked in 1% BSA and incubated with secondary antibody (HRP-conjugated anti-mouse IgG (GE Healthcare), diluted 1∶3000).
Samples were grown for 10 hours at 37°C, fixed, blocked and stained with rabbit-anti-GAS and with Alexa Fluor dye 488 goat anti-rabbit as secondary antibody (Molecular Probes) according to manufacturer's protocol.
For analysis by CLSM, cells were fixed, blocked and stained with rabbit-anti-GAS and with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen Life Technologies, USA).
Specimens were fixed, blocked with 5% normal donkey serum/0.1% Triton-X/PBS for 30 min at room temperature, and exposed to polyclonal goat anti-human Sox17 (R&D systems, AF1924) at a 1∶500 dilution overnight at 4°C.
For immunofluorescence analysis, cells were fixed, blocked, and stained as described above with or without permeabilization.
For dual fluorescent staining, cultures were fixed, blocked and labelled for primary antibodies as observed above.
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