Sentence examples for fixed blocked from inspiring English sources

The podocytes then were fixed, blocked and stained with FITC-labeled phalloidin and DAPI and mounted for microscopy.

Cells were then fixed, blocked in 1% BSA and incubated with secondary antibody (HRP-conjugated anti-mouse IgG (GE Healthcare), diluted 1∶3000).

Samples were grown for 10 hours at 37°C, fixed, blocked and stained with rabbit-anti-GAS and with Alexa Fluor dye 488 goat anti-rabbit as secondary antibody (Molecular Probes) according to manufacturer's protocol.

For analysis by CLSM, cells were fixed, blocked and stained with rabbit-anti-GAS and with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen Life Technologies, USA).

Specimens were fixed, blocked with 5% normal donkey serum/0.1% Triton-X/PBS for 30 min at room temperature, and exposed to polyclonal goat anti-human Sox17 (R&D systems, AF1924) at a 1∶500 dilution overnight at 4°C.

For localization of the endogenous optineurin, rat RGC5 without or with staurosporine treatment and human RPE cells were washed with rinsing solution, fixed, blocked, and double immunostained with rabbit polyclonal C-terminal or INT-optineurin antibody (1∶100, Cayman Chemical, Ann Arbor, MI) and mouse monoclonal anti-GM130 (1∶200, BD Biosciences, San Jose, CA).

Non-independence attributable to multiple measurements on different replicates was therefore dealt with by treating 'replicate' as a fixed blocking factor.

It strikes me that running a big estate is rather similar to being an MP: glamorous from the outside, but the reality is a lot of admin and fixing blocked drains.

In this paper we prove that group divisible 3-designs exist for sufficiently large order with a fixed number of groups, fixed block size and index one, assuming that the necessary arithmetic conditions are satisfied.

There was a time, some years ago, when it was said that the world was moving into a series of fixed blocks.

Other results of the project include the development of new optimization models and algorithms for traffic management, and a general architecture for train traffic control, capable of managing both fixed block and moving block signalling safety concepts.