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At the same time, the living shrimp from which gills had been removed were fixed as appropriate for analysis by light microscopy and TEM.
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Cells were fed and fixed as above.
Generally fix at 0.1 as appropriate for an albedo of 0.3 and solid angle of 0.3.
Statistical analysis: two-way ANOVA or on ranks, as appropriate (fixed effects: inspiratory volume and outcome).
Meta-analysis was performed separately for randomised and non-randomised studies, using either the fixed or random effects model as appropriate.
For polymorphisms assessed in ≥2 studies for the same phenotype assessed with similar case definitions, we conducted fixed or random effects metaanalyses as appropriate using the Metan package (Stata 12.1; StataCorp, College Station, TX).
We also included a fixed or random effect for community, as appropriate.
The prediction models for SGA and LGA were estimated using the estimated logistic regression coefficients and confounders fixed at their means and modes as appropriate [ 8].
Weighted least-squares analyses of the h2 estimates were performed by fitting a number of fixed effects and covariates for each trait as appropriate for the data.
For cell cycle analysis, cells were plated at 80,000 100,000 cells/well, treated as appropriate for 48 hr, fixed, and analyzed by FACS.
VAS scores will be analysed using linear mixed effects models, including fixed effects of time and time dependent covariates as appropriate and random effects of individual.
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