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For the bending experimental set-up three arms were fully clamped at the end accounting for the adhesion to the substrate, while both fixed and sliding boundary conditions were considered for both compression and tensile tests.
These separation techniques provide a large variation in BFI, a difference of 0.741 predicted for BFI in a catchment with the less reliable fixed and sliding interval methods and local minima turning point methods included.
They are a variety of ways to get into the sport, including fixed and sliding seat rowing for beginners of all ages - on rivers, lakes, canals and the coast.
The sections, 40 mm (L) × 1 mm (T) × 20 μm (R), were attached directly to the fixed and sliding aluminum alloy components of the rig using cyanoacrylate adhesive heat-cured for 5 min at 100 °C, taking particular care to achieve accurate axial alignment so that the stress distribution was uniform across the width of the sample.
For vapor-phase deuteration, the fixed and sliding parts of the stretching rig were encased in a purpose-built cell with BaF2 windows built into the top and bottom.
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Transwell inserts were fixed and slides were imaged using a 10× objective on an Olympus BX51 widefield fluorescence microscope and nuclei were manually counted from five fields.
Kidney tissue was fixed and slides were stained with hematoxylin and eosin for routine viewing and by the PAP (peroxidase-antiperoxidase) technique using anti-BrdU to label cells in DNA synthesis.
As for the negative controls, fixed and permeabilized slides were incubated in 50 μL of label solution without TdT.
After 18 hours of co-incubation, oocytes were collected, denuded, washed, fixed, and placed on slides for staining with 0.1% aceto-orcein on slides.
CD138-positive cells were fixed and spun onto slides.
Figure 6: Force displacement curves of a single tetrapod under compression or tension and fixed or sliding boundary conditions as computed by FEM simulations.
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