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The fixed adipose depots were then paraffin embedded, sectioned into 10-μm sections, and processed for hematoxylin and eosin stain.
Morphometric analysis of fixed adipose tissue from the EWAT of wild type and PDZ-RhoGEF KO animals showed no discernable differences in adipocyte size between the genotypes.
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Formalin-fixed adipose and liver tissues were cut in 8-μm sections by Pathology Laboratory Services at McMaster Children's Hospital.
This data indicates that between 5 and 10 days of age in the presence of a positive energy balance, but not a negative energy balance, the capacity for adipose tissue expansion is becoming fixed and will determine the potentiality for adipose tissue expansion in adult mice in an obesogenic environment.
Subcutaneous and visceral adipose tissue was fixed in 4% paraformaldehyde and embedded in paraffin.
Parts of adipose tissue were fixed in 4% formaldehyde in PB buffer for 24 h, washed in PB, dehydrated in ethanol, embedded in paraffin after 2×10 min xylen treatment.
Visceral adipose tissue was fixed and embedded in paraffin.
For histological examination, adipose tissue was fixed in 10% formalin solution and embedded in paraffin.
Immediately after removal, adipose tissues were fixed overnight by immersion at 4°C in 4% paraformaldehyde.
The descending thoracic aorta was removed, cleaned of adipose tissue, and fixed in paraffin wax.
For immunohistological studies, gonadal adipose tissue was fixed in 4% formalin, embedded in paraffin and stained with the corresponding antibodies.
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