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As invagination can clearly be visualized in small ovarian endometrioma without connecting adhesions towards the peritoneum of the lateral pelvic wall, these invaginations and their chocolate content are not due to the fixed adhesion formation preventing endometriotic deposits from escaping, as suggested by Jones et al. [16].
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Gain remained fixed in all adhesion assays.
In this study, it was chosen to apply a fixed time for adhesion and growth which resulted in different surface coverages by adhering bacteria for the different biofilm models (Table 1).
Liver, kidney, spleen, lung, digestive tract and macroscopic tumors (en bloc in the case of severe peritoneal adhesion) were fixed in 10% neutral buffered formalin.
Quantification of cell adhesion: Formaldehyde fixed PCLS were mounted on coverslips using Mowiol containing DABCO (1,4-diazabicyclo-[2.2.2] octane, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) (100 mg/ml).
Cells were fixed and stained for adhesion and proliferation experiments at 2 h, 4 h, 24 and 48 h using DAPI staining.
Cells were fixed and stained for adhesion and for proliferation experiments at 2 h, 4 h, 24 h and 48 h using DAPI staining as described for HAO cells.
It may be difficult to differentiate radiation-induced changes from inflammatory and malignant conditions or adhesions, but fixed bowel loops and the lack of a mass in an area of previous radiotherapy are highly suggestive of radiation enteritis [36].
To analyze the effects of fixative on cellular mechanical properties, we firstly measured adhesion force of fixed and unfixed lymphocytes (Fig. 3a, b).
Breaking free from fixed surface-based biosensing promotes adhesion between the microsphere sensors and the analytes since both can now be thoroughly mixed.
Tumour cells and peripheral blood mononuclear cells were fixed in suspension prior to adhesion to the slide.
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