Exact(2)
After fixation, the position of the needle in the tissue was marked by dipping the tip of the needle (protruding from the end of the tissue) into green ink before pulling it out of the tissue, thus dragging the ink through the tissue as the needle was being pulled back.
After fixation, the position of the microvessel in the mesentery was drawn; the mesentery was cut away from the animal, stored in cacodylate-buffered 4% gluteraldehyde at 4°C overnight, post-fixed in 1% osmium tetroxide, and embedded in araldite blocks using standard techniques.
Similar(58)
Many different techniques have been advocated for the reconstruction of the MPFL, but there is no agreement regarding the choice of graft, the type of fixation, the graft positioning, the correct tension and the clinical outcomes.
By recording the sequence of the fixation positions, the reading behavior can be analyzed and different services provided to the user.
We checked the data statistically by running separate linear regression analysis for the two groups (statistical package R [34]), including the effects of the fixation position (horizontal and vertical) and the viewing distance.
We wondered if the standard deviation of fixation disparity, i.e., the variability in fixation disparity during fixations, was dependent on the position within the text.
The eye-recording system was calibrated with four eccentricities (−10°, −5°, +5°, +10°), to determine the deviation from the fixation position.
When the stimulus is detected, the fixation point moves to the position of the detected stimulus (i.e., the stimulus location becomes the new fixation point).
To prevent the subjects using landmarks to locate the centre dot of the array, the stimulus was randomly perturbed from the fixation point position on each trial independently in x- and y-position from a uniform probability density function with limits ±25 pixels (±25% of the interpatch separation).
Furthermore, the test stimulus could either be shown at the initial fixation position or at the position of the saccade target.
Following fixation, the brain was positioned in the Kopf rat brain blocker (Kopf Instruments PA001) and 2-mm coronal slices were taken in a dorsal-caudal direction.
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