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The method of fixation, the amount of advancement and set back movement, the distraction of the condyle, the displacement of the proximal segment, idiopathic condylar resorption, and the interaction of the para-mandibular connective tissues are considered to be the possible causes of relapse [9 18].
After 24-h co-culture in the examined conditions, the brain slices were washed with PBS and after fixation the amount of fluorescent cells within a slice (i.e. the central planar section) was estimated by means of confocal microscopy.
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We found that the single most influential factor was not extent of fixation, but the amount of time the sample is stored following fixation (See Materials and Methods for details and Fig. S2B, C).
As a consequence of lower carbon fixation by photosynthesis, the amount and percentage of carbon translocated was also lower in heat-stressed fed and unfed nubbins (Ts; Fig. 2a d and Table 2, Fisher's LSD test p < 0.05).
Similar results were obtained when cells were treated with 0.1% Triton X-100 before fixation to reduce the amount of CHD1 that is not bound to chromatin (Figure 1A).
When light declines with depth, without photoacclimation, carbon fixation rates and the amount of translocated carbon declines [ 23].
Variations in ischaemic time or formalin fixation may affect the amount of Ki-67 staining seen in prostate tissue similar to many other IHC assessments (Havelund et al, 2012).
Therefore, we tested the impact of formalin fixation on the arrayed protein and found that this did not impair detection significantly (Fig. 3G); indeed, we noticed an overall stronger signal, possibly due to the cross-linking of proteins during fixation thereby increasing the amount of protein retained on the plate during washing steps.
Besides the number of fixations, dwell time, the amount of time that attention spends at a location once it is deployed to that location [ 56], can also be used as an indicator of the amount of attention paid to a stimulus [ 57].
Average total fixation time refers to the amount of time referees spent fixating various areas of interest within the display prior to making a decision.
Often, in addition to the problems associated with formalin fixation and paraffin embedding, the amount of tissue that is available for nucleic acid isolation is limited.
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