Electron microscopy has sufficient resolution, but the samples have usually been prepared with aldehyde fixation, solvent dehydration, plastic embedment, and heavy-metal staining, which can all introduce artifacts to native structure.
Previously, the use of OCT (time domain) in nystagmus was limited due to fixation instability, poor resolution and slow scanning speeds.
On the other hand, methods based on far field optical microscopy do not require fixation, but the spatial resolution is diffraction limited unless super-resolution techniques are used [ 8].
These observations largely ruled out that these features of higher-order chromatin organization, as seen at the level of 3D-SIM resolution, represent fixation artifacts.
The data obtained on mouse brain sections using TEM are not in accordance with those obtained on cultivated mammalian cells by conventional immunofluorescence technique with the use of different fixation procedures and less microscope resolution.
Fluorescence in situ hybridization (FISH) can provide spatial resolution, but requires sample fixation (eliminating the potential to study a sample over time), extensive preparation steps, and a genetically-targeted exogenous marker.
Second we have applied EDC fixation to improve sensitivity and spatial resolution of the whole mount in situ hybridisation method.
To investigate whether EDC fixation could improve detection and spatial resolution of miRNA expression signals in whole mount in situ hybridization we compared fixation of zebrafish embryos in PFA with PFA+EDC.
ISH was performed in collaboration with the Gene Expression Core Service of Baylor College of Medicine [ 41, 42], but the published protocols using frozen tissue were modified for fixation and embedding to obtain better resolution as follows.
Changes of fixations between snapshots only restrict the resolution with which individual face parts can be processed but don't imply an exclusive processing of the face part fixated on, as would be the case in featural processing.
