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There was an interaction (P<0.05) between the sample source and fixation procedures.
In addition, the functionality of the automatic medium exchange, and fixation procedures were confirmed.
Of all unsuccessful fixation procedures, 42% are caused by failures of the method, sometimes due to mobilization during healing.
We therefore sought to identify optimized fixation procedures which fully leverage the analytical capability of high dimensional flow cytometry without the need for complex cell processing or a sustained cold chain.
The presence, morphology and possible origin of vesicle-like bodies (VBs) within the inner ear otolithic membrane of developmental stages of cichlid fish Oreochromis mossambicus and adult swordtail fish Xiphophorus helleri was analysed by means of transmission and scanning electron microscopy (TEM and SEM, respectively) employing various fixation procedures.
It merits emphasis that correction of predisposing factors and gastric fixation procedures is required to prevent volvulus recurrence.
PMMA injection through fenestrated cannulated screws provided additional stability in fixation procedures carried out on osteoporotic vertebral columns, leading to good pain control in all patients.
All fixation procedures resulted in similar proportions of CLR-immunopositive trigeminal ganglion neurons but both the contrast and regularity of immunostaining showed some differences (Figure 1A-E).
In previous studies the ratio of CLR-immunopositive trigeminal ganglion neurons was reported to exceed the number of RAMP1-positive neurons [19, 20], which was not the case in the present study, possibly due to different fixation procedures or antibodies.
Then, different post fixation procedures were applied.
Fixation procedures are known to disrupt or change the relative geometry of cellular compartments.
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