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This asymmetry cannot be explained by the sequence, which is the same except for the mismatch itself, and probably results from the surface fixation of the probe.
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The anisotropy of probe/target interactions due to the fixation of the probes at the chip surface and end-opening (zippering effects) [13], [49] requires the consideration of the positional dependence of the interactions in a motif-specific fashion, i.e. separately for each NN-combination of nucleotide letters.
Substitution by an appropriate higher-order interaction term which considers the stoichiometry of quadruplex formation, the proximity relations and the fixation of the probes on the chip-surface is expected to modify the respective contribution but leaves the expected trend unchanged.
A principal problem of the developed setup is the need of a freely movable fixation for the probe inside the phantom.
Fixation of the ultrasound flow probe and the catheter with sutures to the musculature made the very fragile system more resilient during transport of the pigs to the scanner room.
This reference scan results in a sensitivity map which presents the sensitivity of the system to the moving glass parts of the probe fixation only.
After achieving correct fusion, the observer pressed the space bar on the computer keyboard to initiate the presentation of the three sine-wave gratings: two test gratings on the left side of fixation, and a probe grating on the right side of fixation, with the initial contrast and phase of the probe grating set randomly.
Throughout each trial, until the onset of the probe, there was a small white square (∼0.8°×0.8°) present at fixation.
b With the fixation restriction, we were able to generate circumferences of 0.3 m applying maximum thrusting force with the maximum inclination in the joystick for different longitudinal orientation of the probe.
b Manual fixation of the expansion tool.
Fixation of the tissues abolished ion fluxes.
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