Sentence examples for fixation of cells from inspiring English sources

This was followed by fixation of cells with ice-cold trichloroacetic acid (50 μl, 50%) at room temperature for 30 min.

After fixation of cells with 100 μl PBS/acetone (2 3 (v/v), Sigma-Aldrich, Bornem, Belgium), cells were washed again.

After 48 h, the media was replaced with 100 µl of 10% Trichloroacetic acid for fixation of cells for 1 h at 4 °C and stained with 0.4% sulforhodamine B dissolved in 1% acetic acid.

Fixation of cells was performed using cold acetone/methanol (50∶50) [14].

Antigen retrieval was performed following fixation of cells with 4% paraformaldehyde.

The comet-like foci became clearly visible by methanol fixation of cells, which removes the spot-like foci (Fig. 2E).

Pre-cooled ethanol:acetic acid (2∶1. v/v) was used for post-fixation of cells on the slide for 5 minutes at −20°C.

Fixations of cells were done following the Apo-BrdU in situ DNA fragmentation assay kit (BioVision, USA) as mentioned previously.

Red cell lysis and fixation of white cells was obtained by Q-prep, and the fluorescence of single cells was measured on an EPICS™ Profile flow cytometer (Coulter).

Cells that had been detached using Cell Dissociation Buffer (Gibco/BRL 13150-016) were allowed to attach to either collagen I, collagen IV, or fibronectin for 10 min before washing to remove nonadherent cells followed by fixation of adherent cells.

Conditioned media derived from G2B-10A cenhancedancolonylony formation of R2-T1AS cells, whereas prior paraformaldehyde (PFA) fixation of G2B-10A cells abrogated this enhancement effect.