Specifically, we varied the colour of the fixation cross, instructions and target boundaries.
To examine whether individuals with Stargardt disease macular dystrophy (STGD) change the location of fixation with instruction.
These responses were made during the fixation block (no instructions were presented).
We manipulated the highly-saturated colour of the instructions, fixation cross and on-screen stimuli.
To assess the effect of chromatic cue on global and local visual processing we introduced a set of chromatic stimulus components (fixation boxes, textual instructions) that could be either red or blue.
In the practice phase, visual information such as instructions, fixation cross, and response feedback was video-projected onto a semitransparent Plexiglas table (size, 70 × 60 cm; height, 84 cm) by a projector (Sharp XR-32X-L), and was therefore available for the participants to monitor.
The first-level design matrix deconstructed the time series into segments corresponding to each of the three cartoon types (11 seconds each), periods of fixation (15 seconds) or instructions (3 s) and inter-stimulus intervals (1 s).
Indeed, 2 of the 5 volunteers who were tested with eye tracking in the current study were unable to consistently suppress eye movements in response to the head turns, even in the presence of a fixation cross and strong instructions to maintain fixation.
Subjects were instructed to simply look at the fixation spot but no other instructions were given (i.e. there was no attentional task).
For phosphatidylserine (PS) detection in vivo, glands were incubated in Annexin-V-Biotin in Ca-binding buffer (TACS Annexin V-Biotin kit, Trevigen 4835-01-K) according to the manufacturer's instructions prior to fixation with 4% PFA, paraffin-embedding and sectioning.
