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"Stay tuned because Pam and I are on a path to do nitrogen fixation in the same sort of way we've just done water splitting," he said.
The eyes were removed and continued with immersion fixation in the same fixative on ice.
Cyanothece sp. ATCC 51142 is a diazotrophic cyanobacterium notable for its ability to perform oxygenic photosynthesis and dinitrogen fixation in the same single cell.
The animals were deeply anesthetized with phenobarbital and perfusion-fixed with 5% formaldehyde in 0.1 M PBS, followed by immersion fixation in the same fixative for 24 h at 4 °C.
Following post fixation in the same fixative overnight, brains were cryoprotected in 30% sucrose, sectioned serially (40 μm), and stored in 50%glycerol/50%% PBS solution at −20 °C until use.
The same laboratory reported that over-expression of Hb1 enhances symbiotic nitrogen fixation in the same system, suggesting that this occurs via removal of NO as an inhibitor of nitrogenase (Shimoda et al. 2009).
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After post-fixation in the same solution overnight, tissue slices were transferred to 2% osmium tetroxide, stained with 2% uranyl acetate, dehydrated and finally embedded in Araldite.
In the simulation run here, a mean of 2.71 fixations per 10 generations were observed in each driver locus for 2 Nσ = 100, but only 0.38 fixations in the same time period for each driver locus for 2 Nσ = 10.
However, the exact alignment of the OVP for any particular word is not precisely defined and may well vary between individuals or even between fixations in the same individual (Rayner, 1998).
The authors have evaluated the patient-reported satisfaction during the treatment period of ring external fixation in this same patient group previously.
All osteotomies healed, and the time in external fixation was the same in both groups: 77 (95% CI: 75 80) days in the zoledronic-treated group and 77 (CI: 74 81) days in the control group.
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