Sentence examples for fixation data not shown from inspiring English sources
First, filaments could be observed in live imaging by the use of N2 gas as well as CO2, and were also observed in flies that were held in CO2 for 10 minutes prior to dissection and immediate fixation (data not shown).
A relatively low value of selection was used under the assumption that the selective force maintaining the cline for the X-4 fusion is stronger than selection on the antagonistic allele, because in simulations where the frequency of the fusion is free to change, the antagonistic allele can drive the fusion to fixation (data not shown).
Infectious virus particles were inactivated by ethanol fixation (data not shown).
In addition, fixation at 12 and 24 hours yielded larger size amplified, antisense RNA (aRNA) molecules, than a 4 h fixation (data not shown).
In E13 14 neocortical walls, we observed Neurog2-d4Venus+ or Gadd45g-d4Venus+ cells in S-phase (labeled with BrdU administrated 20 min before fixation) (data not shown) or M phase (pH3+ or p-Vimentin+) (Fig. 5A,B) of the cell cycle.
As tubulin-like staining of the anti-ADNP antibody also varied with fixation conditions (data not shown), we infer the cytoplasmic patterning of ADNP to be an artifact of the antibody staining procedure.
Only at very low Wolbachia transmission efficiencies does the dominant mutant go to fixation faster (data not shown).
Since most segregation distortions affect the allele frequencies without disturbing the genotypic frequency equilibrium (non significant F value Wright fixation index; data not shown), it is probable that gametic selection was the main factor causing skewed segregation.
The reaction was completely blocked by EDTA (Figure 2B, insert labeled B'), confirming that the DNA degradation was divalent cation-dependent, and no signal was seen upon immediate fixation with paraformaldehyde (data not shown).
All animals showed a specific antibody response detected by the complement fixation test (CFT) (data not shown).
The vascular endothelial marker antibody MECA-32 variably immunostained these vessels under the fixation conditions used (data not shown), and is reported to be variably expressed in tumor blood vessels [ 38].
